Fig. 5

TET-dependent epigenetic activities repress L1 expression. a TET1 depletion leads to a relatively small loss of H3K4me3, whereas TET2-depleted cells have a pronounced loss of H3K9me3 (representative replicate from n = 3–7; see Figure S6B). b ChIP-seq data reveal that OGT and SIN3A, but not EZH2, are enriched at the 5′ UTR of L1Tf elements. c ChIP-qPCR analysis for OGT and SIN3A confirms the binding of both proteins at the 5′ UTR of young L1s (representative replicate from n = 4). d OGT was knocked down in ESCs by siRNA, leading to efficient depletion of the protein, as well as complete loss of cellular O-GlcNAc. e OGT depletion leads to L1 derepression at the RNA level, with partial effects also visible in TET1/OGT double knockdown cells; TE classes that are not TET1 targets are also upregulated (n = 8 for Ogt KD and n = 3 for Tet1/Ogt KD). f SIN3A was knocked down in ESCs by siRNA, with efficient loss of protein expression. g SIN3A depletion leads to L1 derepression at the RNA level, and this depends on TET1 expression; the effect is restricted to TET1-bound TE classes (n = 6 for Sin3a KD and n = 4 for Tet1/Sin3a KD). * p < 0.05, ** p < 0.01, paired t-test comparing to scrambled control