Fig. 5
From: Beyond comparisons of means: understanding changes in gene expression at the single-cell level
Posterior estimates of model parameters for mESCs across different cell-cycle phases. a Empirical distribution of posterior medians for mRNA content normalizing constants \(\phi ^{(p)}_{j}\) across all cells. b Empirical distribution of posterior medians for gene-specific expression rates \(\mu ^{(p)}_{i}\) across all genes. c Empirical distribution of posterior medians for gene-specific biological over-dispersion parameters \(\delta ^{(p)}_{i}\) across all genes. d Upper diagonal panels compare estimates for gene-specific expression rates \(\mu ^{(p)}_{i}\) between groups of cells. Lower diagonal panels compare gene-specific biological over-dispersion parameters \(\delta ^{(p)}_{i}\) between groups of cells. While our results suggest there are no major shifts in mean expression between cell-cycle stages, our results suggest a substantial decrease in biological over-dispersion when cells move from G1 to the S phase, followed by a slight increase after the transition from S to the G2M phase (to give a rough quantification of this statement, panel (d) includes the percentage of point estimates that lie on each side of the diagonal line)